Applications:

Life sciences

Measurements on nanoscale biological samples that are difficult or even impossible using separate imaging modalities

Study of mesenchymal stem cells in collagen scaffold

Understanding cell-scaffold interactions, their relationship to cell morphology, and developing an efficient delivery system for growth factors and other biomolecules to control cell-scaffold signaling are fundamental questions in a wide range of fields. tissue engineering research.

Here, the AFM-in SEM approach made it possible to carry out 3D topography analysis of adherent cells only due to precise navigation of the AFM tip by SEM without damaging the AFM probe in the pores of the stained scaffold.

Benefits of LiteScope:

Fast location of the region of interest and precise navigation of the AFM tip in the complex substrate. CPEM: Detailed topographical analysis, fused with material contrast, showing the interaction of 3D cells with the scaffold under various conditions.

Sample courtesy: Lucy Vojtova, CEITEC BUT, Czech Republic

Topographic analysis of diatom shells

Diatoms are single-celled microalgae found in water and soil around the world. Diatoms, and their shells such as diatomite or diatomaceous earth, are important industrial resources used for fine polishing, liquid filtration, forensic purposes, or nanotechnology.

Benefits of LiteScope:

Fast location of individual diatom shells and accurate AFM tip navigation using SEM. Accurate AFM topographic analysis without damaging the AFM tip.

Sample courtesy: VSB Technical University of Ostrava, Czechia